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1
2018 - 06 - 22
對創(chuàng)傷經歷的回憶會導致精神健康問題,如創(chuàng)傷后應激障礙(PTSD),這會破壞一個人的生活。據(jù)估計,當前將近三分之一的人會在他們生命中的某個時刻遭受恐懼或應激相關的障礙。如今,一項新的研究在細胞水平展示了一種療法如何能夠治療長期的創(chuàng)傷記憶。相關研究結果發(fā)表在2018年6月15日的Science期刊上,論文標題為“Reactivation of recall-induced neurons contributes to remote fear memory attenuation”。論文通信作者、瑞士洛桑聯(lián)邦理工學院(EPFL)教授Johannes Gräff說,“我們的研究結果首次揭示了讓成功治療創(chuàng)傷記憶變得可能的過程。” 在治療創(chuàng)傷記憶領域,對恐懼衰減(fear attenuation)是否涉及通過新的安全記憶痕跡(memory trace of safety)或將原始的恐懼記憶痕跡(memory trace of fear)重寫為安全記憶痕跡來抑制原始的恐懼記憶痕跡,人們長期以來爭論不止。圖片來自EPFL / Gräff Group。這種爭論的一部分與我們總體上還不能完全理解神經元如何存儲記憶的事實相關。雖然這項研究取得的新發(fā)現(xiàn)不能排除這種抑制機制,但是它們首次證實了重寫創(chuàng)傷記憶在治療創(chuàng)傷記憶中的重要性。這個領域的研究重點在于理解大腦減少創(chuàng)傷記憶的能力...
2
2019 - 04 - 12
4 月 9 日,美國紐約市市長比爾 · 德 · 布拉西奧宣布,該市布魯克林區(qū)的威廉斯堡進入緊急狀態(tài),讓當?shù)孛癖娨粫r風聲鶴唳。是什么導致政府宣布這一近年罕見的公共衛(wèi)生緊急情況?答案你或許想不到,肇事者居然是為我們熟知的一種疾病——麻疹。卷土重來,美多地暴發(fā)麻疹疫情麻疹傳染性極強,多見于兒童。我國在 1965 年后進行針對性疫苗接種的推廣,這種疾病發(fā)病率逐漸減少。在美國,本已消失的麻疹病例在近些年時有出現(xiàn),今年在個別地區(qū)更是呈大暴發(fā)之勢。美國疾病控制中心(CDC)數(shù)據(jù)顯示,截至 4 月 4 日,美國 19 個州統(tǒng)計共出現(xiàn) 465 個麻疹病例,成為 2000 年以來疫情第二嚴重年份(2014 年全美病例達 667 例)。而紐約的布魯克林則成為重災區(qū),僅威廉斯堡這一社區(qū)從去年 10 月至今年 4 月 8 日就有 228 例確診病例。除紐約市外,紐約州、新澤西州、華盛頓州、密歇根州以及加州等多個地區(qū)出現(xiàn)了 3 例以上的麻疹病例。而 CDC 將一地區(qū)出現(xiàn) 3 例以上麻疹病例定義為疫情暴發(fā)。一時間,麻疹疫情成為美國公共衛(wèi)生領域最受關注的話題。推波助瀾,“反疫苗運動” 難辭其咎CDC 認為,美國的麻疹疫情與從以色列、烏克蘭和菲律賓等國來美旅客有關,這些國家正在暴發(fā)大規(guī)模麻疹疫情。而對于紐約市布魯克林區(qū)來說,麻疹疫情的暴發(fā)則要歸咎于人們的錯誤認知。一場愈演愈烈的 “反疫苗運動”,...
3
2019 - 08 - 23
2019年8月23日訊 /生物谷BIOON /——2019年6月,歐洲大部分地區(qū)遭遇了早期熱浪,法國的氣溫達到了創(chuàng)紀錄的46攝氏度(115華氏度)。熱浪的特點是在幾天幾夜持續(xù)高溫。它們對我們的日常生活有重要的影響--我們感到過熱和疲勞。當熱浪來襲時,許多政府會啟動一項'熱行動計劃',建議受影響的人多喝水,避免劇烈運動,保持涼爽。如果不這樣做,就有中暑的風險,而中暑有可能危及生命。但是,人體究竟是如何應對如此極端的溫度的呢?為什么高溫如此危險呢?哪些器官受到影響,什么時候情況會變得更糟?圖片來源:http://cn.bing.com保持涼爽人體的核心溫度通常在36到38攝氏度之間波動(97到99華氏度)。在這個溫度范圍內,生化反應可以正常進行,這對細胞和器官的正常功能至關重要。人類的身體也能很好地應對各種可能威脅其核心溫度的環(huán)境條件。當核心溫度偏離正常范圍時,身體就會啟動生理反應,使溫度恢復正常。這種溫度調節(jié)反應相當于你家的恒溫器;一旦內部溫度偏離理想溫度,加熱或冷卻系統(tǒng)就會被激活,回到理想溫度。在人體中,這個恒溫器位于我們大腦的底部,一個叫做下丘腦的區(qū)域。位于我們皮膚、肌肉和其他器官的溫度傳感器提供的信息被分析,必要時,啟動生理反應。對溫度升高的第一個也是最重要的反應是通過我們身體的皮膚和四肢,如手和腳散熱。我們開始產生汗液,汗液會在皮膚上蒸發(fā),在這個過程中散熱。這...
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產品名稱:

Click-iT? EdU Pacific Blue? Flow Cytometry Assay Kit

上市日期: 2019-04-17
產品類別: INVITROGEN

規(guī)格

Flow Cytometer Laser Lines:405
For Use With (Equipment):Flow Cytometer
Detection Method:Fluorescent
Format:Tube(s)
Excitation?Emission (nm):404?450
Label or Dye:Pacific Blue?
Number of Reactions:50
Product Line:Click-iT?,?Pacific Blue?
Product Size:50 assays
Green Features:Less hazardous
Shipping Condition:Room Temperature


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  • 產品功能
  • 產品參數(shù)

描述

The Click-iT? EdU Pacific Blue? Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells as compared to traditional BrdU methods. Newly synthesized DNA is analyzed using the 405 nm laser of the flow cytometer.

??Accurate—superior results compared to BrdU assays
??Fast—results in as little as 90 minutes
??Economical—more assays per kit

View selection guide for all Click-iT? EdU and Click-iT? Plus EdU assays for flow cytometry.

An Advanced Method Giving You Results Superior to BrdU
The most accurate method of proliferation analysis is direct measurement of DNA synthesis. Originally, this was performed through incorporation of radioactive nucleosides, i.e.,?3H-thymidine. This method was replaced by antibody-based detection of the nucleoside analog bromodeoxyuridine (BrdU). The Click-iT? EdU Flow Cytometry Assay Kits are novel alternatives to the BrdU assay. EdU (5-ethynyl-2′-deoxyuridine) is a thymidine analog which is incorporated into DNA during active DNA synthesis. Detection is based on click chemistry: a copper catalyzed covalent reaction between an azide and an alkyne. In this application, the alkyne is found in the ethynyl moiety of EdU, while the azide is coupled to Alexa Fluor ? 488, Alexa Fluor? 647, or Pacific Blue? dyes. Standard flow cytometry methods are used for determining the percentage of S-phase cells in the population (Fig 1).

Mild Conditions Allow Use with Cell Cycle Dyes and Antibodies
The advantages of Click-iT? EdU labeling are readily evident while performing the assay (Fig 2). The small size of the dye azide allows for efficient detection of the incorporated EdU using mild conditions, while standard aldehyde-based fixation and detergent permeabilization are sufficient for the Click-iT? detection reagent to gain access to the DNA. This is in contrast to BrdU assays that require DNA denaturation (using HCl, heat, or digestion with DNase) to expose the BrdU so that it may be detected with an anti-BrdU antibody. Sample processing for the BrdU assay can result in signal alteration of the cell cycle distribution as well as destruction of antigen recognition sites when using the HCl method. In contrast, the easy-to-use EdU cell proliferation kit is compatible with cell cycle dyes. This EdU assay kit can also be multiplexed with antibodies against surface and intracellular markers.

Quick and Simple Protocol
The Click-iT? EdU protocol is based on the aldehyde fixation and detergent permeabilization steps for immunohistochemical antibody labeling, but EdU is compatible with other fixation/permeabilization agents including saponin and methanol. In just five steps you’ll be ready to analyze your cell proliferation data:

? Treat cells with EdUM
? Fix and permeabilize cells
? Detect S-phase cells with Click-iT? detection cocktail for 30 min
? Wash once
? Analyze

Get Accurate Results Economically
By increasing the number of assays per kit, the Click-IT? EdU Pacific Blue? Flow Cytometry Assay Kit is less expensive than the traditional BrdU assays making them ideal for large experiments.

For Research Use Only. Not for use in diagnostic procedures.


規(guī)格

Flow Cytometer Laser Lines:405
For Use With (Equipment):Flow Cytometer
Detection Method:Fluorescent
Format:Tube(s)
Excitation?Emission (nm):404?450
Label or Dye:Pacific Blue?
Number of Reactions:50
Product Line:Click-iT?,?Pacific Blue?
Product Size:50 assays
Green Features:Less hazardous
Shipping Condition:Room Temperature


規(guī)格

Flow Cytometer Laser Lines:405
For Use With (Equipment):Flow Cytometer
Detection Method:Fluorescent
Format:Tube(s)
Excitation?Emission (nm):404?450
Label or Dye:Pacific Blue?
Number of Reactions:50
Product Line:Click-iT?,?Pacific Blue?
Product Size:50 assays
Green Features:Less hazardous
Shipping Condition:Room Temperature


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