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1
2018 - 06 - 22
對創(chuàng)傷經(jīng)歷的回憶會導(dǎo)致精神健康問題,如創(chuàng)傷后應(yīng)激障礙(PTSD),這會破壞一個人的生活。據(jù)估計,當(dāng)前將近三分之一的人會在他們生命中的某個時刻遭受恐懼或應(yīng)激相關(guān)的障礙。如今,一項新的研究在細(xì)胞水平展示了一種療法如何能夠治療長期的創(chuàng)傷記憶。相關(guān)研究結(jié)果發(fā)表在2018年6月15日的Science期刊上,論文標(biāo)題為“Reactivation of recall-induced neurons contributes to remote fear memory attenuation”。論文通信作者、瑞士洛桑聯(lián)邦理工學(xué)院(EPFL)教授Johannes Gräff說,“我們的研究結(jié)果首次揭示了讓成功治療創(chuàng)傷記憶變得可能的過程。” 在治療創(chuàng)傷記憶領(lǐng)域,對恐懼衰減(fear attenuation)是否涉及通過新的安全記憶痕跡(memory trace of safety)或?qū)⒃嫉目謶钟洃浐圹E(memory trace of fear)重寫為安全記憶痕跡來抑制原始的恐懼記憶痕跡,人們長期以來爭論不止。圖片來自EPFL / Gräff Group。這種爭論的一部分與我們總體上還不能完全理解神經(jīng)元如何存儲記憶的事實相關(guān)。雖然這項研究取得的新發(fā)現(xiàn)不能排除這種抑制機(jī)制,但是它們首次證實了重寫創(chuàng)傷記憶在治療創(chuàng)傷記憶中的重要性。這個領(lǐng)域的研究重點在于理解大腦減少創(chuàng)傷記憶的能力...
2
2019 - 04 - 12
4 月 9 日,美國紐約市市長比爾 · 德 · 布拉西奧宣布,該市布魯克林區(qū)的威廉斯堡進(jìn)入緊急狀態(tài),讓當(dāng)?shù)孛癖娨粫r風(fēng)聲鶴唳。是什么導(dǎo)致政府宣布這一近年罕見的公共衛(wèi)生緊急情況?答案你或許想不到,肇事者居然是為我們熟知的一種疾病——麻疹。卷土重來,美多地暴發(fā)麻疹疫情麻疹傳染性極強(qiáng),多見于兒童。我國在 1965 年后進(jìn)行針對性疫苗接種的推廣,這種疾病發(fā)病率逐漸減少。在美國,本已消失的麻疹病例在近些年時有出現(xiàn),今年在個別地區(qū)更是呈大暴發(fā)之勢。美國疾病控制中心(CDC)數(shù)據(jù)顯示,截至 4 月 4 日,美國 19 個州統(tǒng)計共出現(xiàn) 465 個麻疹病例,成為 2000 年以來疫情第二嚴(yán)重年份(2014 年全美病例達(dá) 667 例)。而紐約的布魯克林則成為重災(zāi)區(qū),僅威廉斯堡這一社區(qū)從去年 10 月至今年 4 月 8 日就有 228 例確診病例。除紐約市外,紐約州、新澤西州、華盛頓州、密歇根州以及加州等多個地區(qū)出現(xiàn)了 3 例以上的麻疹病例。而 CDC 將一地區(qū)出現(xiàn) 3 例以上麻疹病例定義為疫情暴發(fā)。一時間,麻疹疫情成為美國公共衛(wèi)生領(lǐng)域最受關(guān)注的話題。推波助瀾,“反疫苗運(yùn)動” 難辭其咎CDC 認(rèn)為,美國的麻疹疫情與從以色列、烏克蘭和菲律賓等國來美旅客有關(guān),這些國家正在暴發(fā)大規(guī)模麻疹疫情。而對于紐約市布魯克林區(qū)來說,麻疹疫情的暴發(fā)則要?dú)w咎于人們的錯誤認(rèn)知。一場愈演愈烈的 “反疫苗運(yùn)動”,...
3
2019 - 08 - 23
2019年8月23日訊 /生物谷BIOON /——2019年6月,歐洲大部分地區(qū)遭遇了早期熱浪,法國的氣溫達(dá)到了創(chuàng)紀(jì)錄的46攝氏度(115華氏度)。熱浪的特點是在幾天幾夜持續(xù)高溫。它們對我們的日常生活有重要的影響--我們感到過熱和疲勞。當(dāng)熱浪來襲時,許多政府會啟動一項'熱行動計劃',建議受影響的人多喝水,避免劇烈運(yùn)動,保持涼爽。如果不這樣做,就有中暑的風(fēng)險,而中暑有可能危及生命。但是,人體究竟是如何應(yīng)對如此極端的溫度的呢?為什么高溫如此危險呢?哪些器官受到影響,什么時候情況會變得更糟?圖片來源:http://cn.bing.com保持涼爽人體的核心溫度通常在36到38攝氏度之間波動(97到99華氏度)。在這個溫度范圍內(nèi),生化反應(yīng)可以正常進(jìn)行,這對細(xì)胞和器官的正常功能至關(guān)重要。人類的身體也能很好地應(yīng)對各種可能威脅其核心溫度的環(huán)境條件。當(dāng)核心溫度偏離正常范圍時,身體就會啟動生理反應(yīng),使溫度恢復(fù)正常。這種溫度調(diào)節(jié)反應(yīng)相當(dāng)于你家的恒溫器;一旦內(nèi)部溫度偏離理想溫度,加熱或冷卻系統(tǒng)就會被激活,回到理想溫度。在人體中,這個恒溫器位于我們大腦的底部,一個叫做下丘腦的區(qū)域。位于我們皮膚、肌肉和其他器官的溫度傳感器提供的信息被分析,必要時,啟動生理反應(yīng)。對溫度升高的第一個也是最重要的反應(yīng)是通過我們身體的皮膚和四肢,如手和腳散熱。我們開始產(chǎn)生汗液,汗液會在皮膚上蒸發(fā),在這個過程中散熱。這...
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產(chǎn)品名稱:

Premo? FUCCI Cell Cycle Sensor (BacMam 2.0)

上市日期: 2019-04-18
產(chǎn)品類別: INVITROGEN

規(guī)格

Color:Green,?Red-Orange
Delivery Method:Baculovirus (BacMam System)
Detection Method:Fluorescent
Emission Class:Visible
Excitation Class:Visible
Excitation?Emission (nm):488?510 (Green),?555?584 (Red)
For Use With (Equipment):Flow Cytometer,?Fluorescence Microscope,?Fluorescent Imager,?High Content Instrument,?Tali? Image-Based Cytometer
Form:Liquid
Label or Dye:GFP (EmGFP),?RFP (TagRFP)
Product Line:Premo?
Product Size:2 x 200 μL
Quantity:2 vials (200 μl each)
Sub-Cellular Localization:Nucleus
Technique:Fluorescence Intensity
Shipping Condition:Wet Ice


  • 產(chǎn)品描述
  • 產(chǎn)品功能
  • 產(chǎn)品參數(shù)

描述

Premo? FUCCI Cell Cycle Sensor is a fluorescent, two-color sensor of cell cycle progression and division in live cells. It allows accurate and sensitive cell cycle analysis of individual cells or a population of cells by fluorescence microscopy, flow cytometry, or high-content imaging.

At 2 x 200 μL, this is a smaller pack size version (1/5 volume) of the popular full-size product and perfect for testing its performance in your system.

The Premo? FUCCI Cell Cycle Sensor is delivered by highly efficient BacMam 2.0 technology, enabling cell cycle studies in essentially any cell type. Provided in a ready-to-use format—simply add, incubate, and image. Premo? FUCCI affords highly efficient transient expression in cell lines, primary cells, and stem cells.

Premo? FUCCI Cell Cycle Sensor is:

? Accurate: Cell-cycle controlled expression of bright GFP and RFP indicators for live cell analysis of individual cells or populations
? Highly Efficient: >90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
? Fast and Convenient: Simply add Premo? FUCCI Cell Cycle Sensor to your cells in complete medium, incubate overnight, and analyze
? Safe: BacMam reagents are non-integrating and non-replicating in mammalian cells, lack observable cytopathic effects, and are suitable for biosafety level (BSL) 1 handling

Study Cell Cycle in the Relevant Cellular Context
If you are studying cell cycle regulators, cell differentiation, or are developing anti-cancer compounds against cell cycle-related targets, Premo? FUCCI is a superbly sensitive and accurate sensor tool. The efficient transduction and transient expression allows you to conduct these experiments in the biologically relevant cell types.

Simple, Quick Cell Cycle Visualization
Simply add the ready-to-use Premo? FUCCI cell cycle sensor to your cells in complete medium, incubate overnight, then visualize cell cycle progression in populations of cells using fluorescence microscopy, flow cytometry, or high content imaging instruments. Cells change from red in the G1 to yellow in the G1/S interphase and green in S, G2, and M phases, as fusions of emGFP and TagRFP coupled to two cell cycle-regulated proteins are expressed and degraded.

How It Works
Premo? FUCCI is derived from the fluorescence ubiquitination cell cycle indicator (FUCCI), developed by Miyawaki and colleagues (Ref 1). FUCCI is based on two cell cycle-regulated proteins, geminin and Cdt1, fused to one green (emGFP) and red (TagRFP) fluorescent protein, respectively. As Cdt1 and geminin are present only during specific phases of the cell cycle, the fluorescent protein partner is similarly cell-cycle dependent. Ubiquitination by specific ubiquitin E3 ligases target the fusion constructs in the proteasome for degradation. E3 ligases display temporal regulation of activity, resulting in biphasic cycling of geminin and Cdt1 levels during the cell cycle. Geminin-GFP is degraded in the G1 phase; the presence of Cdt1-TagRFP is indicated by red fluorescence within nuclei. During the S, G2, and M phases, however, Cdt1 is degraded and only geminin-GFP remains, giving cells green-fluorescent nuclei. During the G1/S transition, when Cdt1 levels are decreasing and geminin levels are increasing, both proteins are present, giving a yellow-fluorescent nuclear signal. This dynamic color change (red to yellow to green) clearly displays progression through the cell cycle and division.

Powered by BacMam 2.0 Technology
BacMam technology is based on an insect virus (baculovirus) for efficient transduction and transient expression in mammalian cells. The Premo? FUCCI cell cycle sensor uses BacMam 2.0 technology to enable cell cycle studies in essentially any cell type, from cell lines to stem cells by incorporation of a mammalian expression cassette for the FUCCI construct. Compared with BacMam 1.0 technology, BacMam 2.0 does not require the use of an enhancer and can be used in complete medium. There is no need to purify plasmid or worry about vector integrity and quality. No lipids, dye-loading chemicals, or other potentially harmful treatments are required. BacMam 2.0 incorporates elements that greatly enhance transduction efficiency and expression levels: a pseudotyped capsid protein for more efficient cell entry, an enhanced CMV promoter, and the Woodchuck hepatitis post-transcriptional regulatory element (WPRE).

Titratable Expression that Lasts Up to Two Weeks
The transient transgene expression lasts from about 5 days in transformed cell lines to up to two weeks in slowly dividing cells, such as some primary cell types; we have imaged terminally differentiated neurons more than three weeks after transduction. In addition, BacMam technology permits defined optimization because it gives you the ability to precisely titrate expression levels. Baculoviruses do not replicate in mammalian cells, nor are viral genes expressed, giving them an excellent safety profile and lack of cytopathic effects on mammalian cells.

Learn more about the Premo? FUCCI Cell Cycle Sensor

Reference:

1. Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A. Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell. 2008 Feb 8;132(3):487-98. PubMed PMID: 18267078

Related products
We offer a range of BacMam-based reagents beyond CellLight? reagents, including the BacMam GFP Transduction Control that is ideal to test out the technology and optimize transduction conditions, Premo? Biosensors, including Premo? Autophagy Sensor, ion channel drug targets, pathway analysis kits, and more.

Learn more about these products and the BacMam technology

See other imaging tools and reagents

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use


規(guī)格

Color:Green,?Red-Orange
Delivery Method:Baculovirus (BacMam System)
Detection Method:Fluorescent
Emission Class:Visible
Excitation Class:Visible
Excitation?Emission (nm):488?510 (Green),?555?584 (Red)
For Use With (Equipment):Flow Cytometer,?Fluorescence Microscope,?Fluorescent Imager,?High Content Instrument,?Tali? Image-Based Cytometer
Form:Liquid
Label or Dye:GFP (EmGFP),?RFP (TagRFP)
Product Line:Premo?
Product Size:2 x 200 μL
Quantity:2 vials (200 μl each)
Sub-Cellular Localization:Nucleus
Technique:Fluorescence Intensity
Shipping Condition:Wet Ice


規(guī)格

Color:Green,?Red-Orange
Delivery Method:Baculovirus (BacMam System)
Detection Method:Fluorescent
Emission Class:Visible
Excitation Class:Visible
Excitation?Emission (nm):488?510 (Green),?555?584 (Red)
For Use With (Equipment):Flow Cytometer,?Fluorescence Microscope,?Fluorescent Imager,?High Content Instrument,?Tali? Image-Based Cytometer
Form:Liquid
Label or Dye:GFP (EmGFP),?RFP (TagRFP)
Product Line:Premo?
Product Size:2 x 200 μL
Quantity:2 vials (200 μl each)
Sub-Cellular Localization:Nucleus
Technique:Fluorescence Intensity
Shipping Condition:Wet Ice


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