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2018 - 06 - 22
對創(chuàng)傷經(jīng)歷的回憶會導(dǎo)致精神健康問題,如創(chuàng)傷后應(yīng)激障礙(PTSD),這會破壞一個人的生活。據(jù)估計,當(dāng)前將近三分之一的人會在他們生命中的某個時刻遭受恐懼或應(yīng)激相關(guān)的障礙。如今,一項新的研究在細胞水平展示了一種療法如何能夠治療長期的創(chuàng)傷記憶。相關(guān)研究結(jié)果發(fā)表在2018年6月15日的Science期刊上,論文標(biāo)題為“Reactivation of recall-induced neurons contributes to remote fear memory attenuation”。論文通信作者、瑞士洛桑聯(lián)邦理工學(xué)院(EPFL)教授Johannes Gräff說,“我們的研究結(jié)果首次揭示了讓成功治療創(chuàng)傷記憶變得可能的過程。” 在治療創(chuàng)傷記憶領(lǐng)域,對恐懼衰減(fear attenuation)是否涉及通過新的安全記憶痕跡(memory trace of safety)或?qū)⒃嫉目謶钟洃浐圹E(memory trace of fear)重寫為安全記憶痕跡來抑制原始的恐懼記憶痕跡,人們長期以來爭論不止。圖片來自EPFL / Gräff Group。這種爭論的一部分與我們總體上還不能完全理解神經(jīng)元如何存儲記憶的事實相關(guān)。雖然這項研究取得的新發(fā)現(xiàn)不能排除這種抑制機制,但是它們首次證實了重寫創(chuàng)傷記憶在治療創(chuàng)傷記憶中的重要性。這個領(lǐng)域的研究重點在于理解大腦減少創(chuàng)傷記憶的能力...
2
2019 - 04 - 12
4 月 9 日,美國紐約市市長比爾 · 德 · 布拉西奧宣布,該市布魯克林區(qū)的威廉斯堡進入緊急狀態(tài),讓當(dāng)?shù)孛癖娨粫r風(fēng)聲鶴唳。是什么導(dǎo)致政府宣布這一近年罕見的公共衛(wèi)生緊急情況?答案你或許想不到,肇事者居然是為我們熟知的一種疾病——麻疹。卷土重來,美多地暴發(fā)麻疹疫情麻疹傳染性極強,多見于兒童。我國在 1965 年后進行針對性疫苗接種的推廣,這種疾病發(fā)病率逐漸減少。在美國,本已消失的麻疹病例在近些年時有出現(xiàn),今年在個別地區(qū)更是呈大暴發(fā)之勢。美國疾病控制中心(CDC)數(shù)據(jù)顯示,截至 4 月 4 日,美國 19 個州統(tǒng)計共出現(xiàn) 465 個麻疹病例,成為 2000 年以來疫情第二嚴重年份(2014 年全美病例達 667 例)。而紐約的布魯克林則成為重災(zāi)區(qū),僅威廉斯堡這一社區(qū)從去年 10 月至今年 4 月 8 日就有 228 例確診病例。除紐約市外,紐約州、新澤西州、華盛頓州、密歇根州以及加州等多個地區(qū)出現(xiàn)了 3 例以上的麻疹病例。而 CDC 將一地區(qū)出現(xiàn) 3 例以上麻疹病例定義為疫情暴發(fā)。一時間,麻疹疫情成為美國公共衛(wèi)生領(lǐng)域最受關(guān)注的話題。推波助瀾,“反疫苗運動” 難辭其咎CDC 認為,美國的麻疹疫情與從以色列、烏克蘭和菲律賓等國來美旅客有關(guān),這些國家正在暴發(fā)大規(guī)模麻疹疫情。而對于紐約市布魯克林區(qū)來說,麻疹疫情的暴發(fā)則要歸咎于人們的錯誤認知。一場愈演愈烈的 “反疫苗運動”,...
3
2019 - 08 - 23
2019年8月23日訊 /生物谷BIOON /——2019年6月,歐洲大部分地區(qū)遭遇了早期熱浪,法國的氣溫達到了創(chuàng)紀錄的46攝氏度(115華氏度)。熱浪的特點是在幾天幾夜持續(xù)高溫。它們對我們的日常生活有重要的影響--我們感到過熱和疲勞。當(dāng)熱浪來襲時,許多政府會啟動一項'熱行動計劃',建議受影響的人多喝水,避免劇烈運動,保持涼爽。如果不這樣做,就有中暑的風(fēng)險,而中暑有可能危及生命。但是,人體究竟是如何應(yīng)對如此極端的溫度的呢?為什么高溫如此危險呢?哪些器官受到影響,什么時候情況會變得更糟?圖片來源:http://cn.bing.com保持涼爽人體的核心溫度通常在36到38攝氏度之間波動(97到99華氏度)。在這個溫度范圍內(nèi),生化反應(yīng)可以正常進行,這對細胞和器官的正常功能至關(guān)重要。人類的身體也能很好地應(yīng)對各種可能威脅其核心溫度的環(huán)境條件。當(dāng)核心溫度偏離正常范圍時,身體就會啟動生理反應(yīng),使溫度恢復(fù)正常。這種溫度調(diào)節(jié)反應(yīng)相當(dāng)于你家的恒溫器;一旦內(nèi)部溫度偏離理想溫度,加熱或冷卻系統(tǒng)就會被激活,回到理想溫度。在人體中,這個恒溫器位于我們大腦的底部,一個叫做下丘腦的區(qū)域。位于我們皮膚、肌肉和其他器官的溫度傳感器提供的信息被分析,必要時,啟動生理反應(yīng)。對溫度升高的第一個也是最重要的反應(yīng)是通過我們身體的皮膚和四肢,如手和腳散熱。我們開始產(chǎn)生汗液,汗液會在皮膚上蒸發(fā),在這個過程中散熱。這...
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產(chǎn)品名稱:

LIVE/DEAD? Fixable Green Dead Cell Stain Kit, for 488 nm excitation

上市日期: 2019-04-22
產(chǎn)品類別: INVITROGEN

規(guī)格

Compatible Cells:Eukaryotic Cells
For Use With (Equipment):Flow Cytometer
Detection Method:Fluorescent
Format:Tube(s)
Excitation?Emission (nm):495?520
Flow Cytometer Laser Lines:488
Form:Solid
Label or Dye:LIVE/DEAD? Fixable Green Dead Cell Stain
Number of Reactions:200
Product Line:LIVE/DEAD?
Product Size:200 assays
Solubility:DMSO (Dimethylsulfoxide)
Shipping Condition:Room Temperature


  • 產(chǎn)品描述
  • 產(chǎn)品功能
  • 產(chǎn)品參數(shù)

描述

The LIVE/DEAD? Fixable Green Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. This kit has been optimized and validated for use with a blue laser flow cytometer.

? Stable—dyes are freeze dried in separate vials to maintain stability
? Robust—staining pattern is the same before and after fixation
? Bright signal—allows for easy distinction between live/dead cells in single channel

View a selection guide for all fixable viability dyes for flow cytometry.

Stable
Unlike products that are sold in solution, the LIVE/DEAD? Fixable Green Stain has been conveniently packaged in 40-test vials to help ensure the stability and performance of the dye over time. Amine reactive dyes in solution will lose their effectiveness over a short period of time, therefore it is recommended to completely use the vial once rehydrated. If this is not possible, aliquot the vials in small volumes and store at -80°C, avoiding freeze-thaw cycles.

Robust
Dead cell discriminator stains can lose sensitivity after treatment with fixatives such as formaldehyde or ethanol-based fixation methods required for intracellular phosphorylation studies. The LIVE/DEAD? Fixable Green Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines, and the staining pattern is preserved following formaldehyde fixation.

Optimal brightness
The LIVE/DEAD? Fixable Green stain was selected based on its fluorescent properties to provide a bright signal when excited with a blue 488 nm laser. The green-fluorescent reactive dye has an excitation maximum of ~495 nm making it ideal for use with the blue laser and an emission of ~520 nm allowing collection in the FITC channel. Since live and dead cells can be discriminated using a single dye and a single channel of a flow cytometer, it is an ideal choice for multi-color experiments.

How it works
In cells with compromised membranes, the dye reacts with free amines both in the cell interior and on the cell surface, yielding intense fluorescent staining. In viable cells, the dye's reactivity is restricted to the cell-surface amines, resulting in less intense fluorescence. The difference in intensity is typically greater than 50-fold between live and dead cells, allowing for easy discrimination.

Colors available
LIVE/DEAD? Fixable Dead Cell Stains are available in a wide variety of colors to meet your multi-color panel needs.


規(guī)格

Compatible Cells:Eukaryotic Cells
For Use With (Equipment):Flow Cytometer
Detection Method:Fluorescent
Format:Tube(s)
Excitation?Emission (nm):495?520
Flow Cytometer Laser Lines:488
Form:Solid
Label or Dye:LIVE/DEAD? Fixable Green Dead Cell Stain
Number of Reactions:200
Product Line:LIVE/DEAD?
Product Size:200 assays
Solubility:DMSO (Dimethylsulfoxide)
Shipping Condition:Room Temperature


規(guī)格

Compatible Cells:Eukaryotic Cells
For Use With (Equipment):Flow Cytometer
Detection Method:Fluorescent
Format:Tube(s)
Excitation?Emission (nm):495?520
Flow Cytometer Laser Lines:488
Form:Solid
Label or Dye:LIVE/DEAD? Fixable Green Dead Cell Stain
Number of Reactions:200
Product Line:LIVE/DEAD?
Product Size:200 assays
Solubility:DMSO (Dimethylsulfoxide)
Shipping Condition:Room Temperature


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