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1
2019 - 02 - 13
日前，《分子植物》（Molecular Plant）在線發(fā)表了中國農(nóng)業(yè)科學(xué)院油料作物研究所王漢中院士團隊最新成果。該團隊在國際上首次成功克隆了農(nóng)作物種子性狀的第一個細(xì)胞質(zhì)調(diào)控基因 orf188，并揭示了該基因調(diào)控油菜種子高含油量的作用機制。該成果為油菜高含油量育種提供了新途徑，為育種過程中雜交母本的選擇提供了理論支撐，對于農(nóng)作物不同類型細(xì)胞質(zhì)的應(yīng)用具有重要的指導(dǎo)意義。含油量是油菜產(chǎn)油量最重要的構(gòu)成因子之一，隨著現(xiàn)代育種對含油量潛力的不斷挖掘，提高品種的含油量越來越困難。該團隊在油菜含油量遺傳研究的基礎(chǔ)上，從細(xì)胞質(zhì)效應(yīng)著手對含油量母體調(diào)控新機制研究取得突破，篩選到兩個對含油量具有極顯著細(xì)胞質(zhì)正負(fù)效應(yīng)的品種，鑒定出特異基因 orf188 控制細(xì)胞質(zhì)的含油量正效應(yīng)，過表達(dá)該基因可以大幅度提升含油量。研究表明，orf188 為 nap（nap-like）型油菜品種所特有基因，是一個新近進化形成的嵌合基因。該團隊十余年來圍繞油菜種子性狀的母體調(diào)控進行系統(tǒng)研究，此次對細(xì)胞質(zhì)基因調(diào)控種子含油量機理的揭示，是該團隊繼發(fā)現(xiàn)角果皮發(fā)育調(diào)控種子粒重和含油量之后發(fā)現(xiàn)的又一條母體調(diào)控新途徑，進一步豐富和完善了種子性狀的母體調(diào)控理論，也為其他農(nóng)作物性狀細(xì)胞質(zhì)效應(yīng)的解析提供了借鑒。相關(guān)論文信息：DOI:10.1016/j.molp.2019.01.012來源：中國科學(xué)報轉(zhuǎn)載：生物360
2
2019 - 06 - 14
隨著技術(shù)飛速發(fā)展、醫(yī)學(xué)數(shù)據(jù)的持續(xù)擴增以及硬件設(shè)備的不斷提升，人工智能和醫(yī)療的結(jié)合方式越來越多樣化。目前AI在醫(yī)療領(lǐng)域中的落地的應(yīng)用場景主要有醫(yī)學(xué)影像、智能診療、智能導(dǎo)診、智能語音、健康管理、病例分析、醫(yī)院管理、新藥研發(fā)和醫(yī)療機器人等，其中在醫(yī)學(xué)影像中的應(yīng)用最為廣泛。一、影像醫(yī)學(xué)發(fā)展現(xiàn)狀醫(yī)學(xué)影像是醫(yī)生完成診斷的主要依據(jù)，通過對影像的分析和比較，從而完成有依據(jù)的診斷。但是在實際過程中，往往會存在以下問題：（1）影像學(xué)診斷人才資源緊缺。醫(yī)療機構(gòu)普遍缺乏高水平的影像醫(yī)師，在疾病診斷時往往會發(fā)生同病異影，異病同影等情況。（2）傳統(tǒng)定性分析存在診斷誤差。醫(yī)生普遍擅長定性分析，很多微小的定量變化無法通過肉眼判斷，很難做到定量分析。（3）醫(yī)生閱片時間長。目前的影像呈現(xiàn)方式為數(shù)據(jù)和圖像，而不是最有效的信息，很大程度上限制了醫(yī)生的人工閱片速度。二、AI+醫(yī)學(xué)影像助力疾病診斷通過引入人工智能可有效解決部分問題，目前人工智能在醫(yī)學(xué)影像領(lǐng)域的應(yīng)用方向主要以下幾類：1. 影像設(shè)備的圖像重建AI可以通過算法的圖像映射技術(shù)，將采集的少量信號恢復(fù)出與全采樣圖像同樣質(zhì)量的圖像，而且使用圖像重建技術(shù)，可以由低劑量的CT和PET圖像重建得到高劑量質(zhì)量圖像。這樣在滿足臨床診斷需求的同時，還能夠降低輻射的風(fēng)險。2. 智能輔助診斷疾病（1）智能輔助診斷肺部疾病國內(nèi)應(yīng)用AI+CT影像最為成熟的領(lǐng)域在肺結(jié)節(jié)的識別上。AI能夠有效識...
3
2019 - 12 - 25
前瞻記憶是指個體記住未來要完成任務(wù)的能力。兒童進入學(xué)校后，前瞻記憶任務(wù)逐漸增多，對前瞻記憶能力的要求也逐漸增高。前瞻記憶任務(wù)成功的關(guān)鍵在于當(dāng)個體看到線索時能主動提取并執(zhí)行意圖。前瞻記憶線索分為三種，時間線索（如周六晚上7點記得參加朋友的生日聚會）、事件線索（如路過超市記得買水果）和活動結(jié)束線索（如寫完作業(yè)記得找家長簽字）。三種線索的提取難度存在差異，這可能與其對注意資源的需求不同有關(guān)。較之正常發(fā)育的兒童，多動癥兒童在日?；顒又谐Ｓ凶⒁饬Σ患泻徒⊥谋憩F(xiàn)，提示其前瞻記憶能力可能存在缺損。然而，對多動癥兒童前瞻記憶功能的研究較少，更缺乏研究直接考察多動癥兒童在三種線索條件下的前瞻記憶表現(xiàn)及其認(rèn)知加工特點。因此，中國科學(xué)院心理研究所心理健康重點實驗室神經(jīng)心理和應(yīng)用認(rèn)知神經(jīng)科學(xué)（NACN）實驗室陳楚僑研究組與北醫(yī)六院醫(yī)生錢英、濰坊醫(yī)學(xué)院教授王艷郁合作考察了多動癥兒童在時間、事件和活動結(jié)束線索條件下的前瞻記憶表現(xiàn)。該研究招募了28名臨床診斷的多動癥兒童和28名年齡和性別匹配的正常發(fā)育兒童，采用釣魚游戲任務(wù)(Yang， Chan， Shum， 2011)測查兒童在三種線索條件下的前瞻記憶功能。該任務(wù)采取經(jīng)典的雙任務(wù)范式，主任務(wù)要求兒童釣魚，并同時關(guān)注前瞻記憶線索，在線索出現(xiàn)時兒童需要停止釣魚并執(zhí)行前瞻記憶任務(wù)。在時間線索條件下，兒童要記得每到整分鐘就喂貓吃魚；在事件線索條件下，兒童看到特殊條...
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時間： 2019 - 04 - 22
描述The LIVE/DEAD? Fixable Green Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. This kit has been optimized and validated for use with a blue laser flow cytometer.? Stable—dyes are freeze dried in separate vials to maintain stability? Robust—staining pattern is the same before and after fixation? Bright signal—allows...
型號：
時間： 2019 - 04 - 18
描述This product detects the externalization of phosphatidylserine in apoptotic cells using recombinant annexin V conjugated to green-fluorescent FITC dye and dead cells using propidium iodide (PI). Propidium iodide stains necrotic cells with red fluorescence. After treatment with both probes, apoptotic cells show green fluorescence, dead cells show red and green fluorescence, and live cells show little or no fluorescence.
型號：
時間： 2019 - 04 - 18
描述SYTOX® Green nucleic acid stain is an excellent green-fluorescent nuclear and chromosome counterstain that is impermeant to live cells, making it a useful indicator of dead cells within a population.?  Impermeant to live cells?  Excitation/Emission: 504/523 nm?  Use with 488 Argon-ion laser?  Works with mammalian cells and both Gram-positive and Gram-negative bacteriaSimple Viability Determination SYTOX® Green allows quick determination of cell viability when usin...
型號：
時間： 2019 - 04 - 18
描述Premo? FUCCI Cell Cycle Sensor is a fluorescent, two-color sensor of cell cycle progression and division in live cells. It allows accurate and sensitive cell cycle analysis of individual cells or a population of cells by fluorescence microscopy, flow cytometry, or high-content imaging.At 2 x 200 µL, this is a smaller pack size version (1/5 volume) of the popular full-size product and perfect for testing its performance in your system.The Premo? FUCCI Cell Cycle Sensor is delivered by high...
型號：
時間： 2019 - 04 - 18
描述CountBright? absolute counting beads are a calibrated suspension of microspheres that are brightly fluorescent across a wide range of excitation and emission wavelengths (UV to 635 nm excitation and 385-800 nm emission). CountBright? absolute counting beads are mixed with the cell sample and assayed via flow cytometry. By comparing the ratio of bead events to cell events, absolute numbers of cells in the sample can be calculated. Because CountBright? beads are mixed in the test sample, absolut...
型號：
時間： 2019 - 04 - 17
描述CellTrace? CFSE Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry.? Superior performance—bright, single-peak staining enables visualization of multiple generations? Long-term signal stability—well-retained in cells for several days post stain? Non-toxic—staining does not adversely effect cell health? Simple, robust staining protocolView a selection guide for all CellTrace? Cell Proliferation Kits fo...
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時間： 2019 - 04 - 17
描述CellTrace? CFSE 細(xì)胞增殖試劑盒 (CellTrace? CFSE Cell Proliferation Kit) 以方便的一次性瓶裝形式提供用途廣泛，穩(wěn)定性高的細(xì)胞示蹤劑。 羧基熒光素二醋酸鹽琥珀酰亞胺酯，常稱為 CFSE，可被動擴散進入細(xì)胞。 這種試劑沒有顏色和熒光，直至細(xì)胞內(nèi)的酯酶去除其醋酸鹽基團，產(chǎn)生能發(fā)出明亮熒光的羧基熒光素琥珀酰亞胺酯。 琥珀酰亞胺酯與細(xì)胞內(nèi)氨基反應(yīng)，形成穩(wěn)定且能用醛固定劑固定的熒光偶聯(lián)物。 此試劑盒包含十份一次性瓶裝的 CFSE，因此進行小規(guī)模實驗時無需制備過量的易變質(zhì) CFSE 儲存液。 此試劑盒還包括高質(zhì)量的 DMSO（二甲基亞砜）和詳細(xì)的實驗方案。For Research Use Only. Not for use in diagnostic procedures.圖表Suppressive capacity of isolated Treg cells.Suppressive capacity of isolated Treg cells. (A) CD4+ CD25–cells were stained with Ca...
型號：
時間： 2019 - 04 - 17
描述The Click-iT® EdU Pacific Blue? Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells as compared to traditional BrdU methods. Newly synthesized DNA is analyzed using the 405 nm laser of the flow cytometer.? Accurate—superior results compared to BrdU assays? Fast—results in as little as 90 minutes? Economical—more assays per kitView selection guide for all Click-iT? EdU and Click-iT? Plus EdU assays for flow c...
型號：
時間： 2019 - 04 - 17
描述The 2018 Attune NxT Acoustic Focusing Cytometer has expanded functionalities to drive your research forward. Designed and developed to remove common barriers associated with flow cytometry, the evolutionary capabilities of the Attune NxT flow cytometer offer adaptable optical configuration options so you can get the most out of your multicolor analysis.? Free Attune NxT software with researcher-inspired upgrades? New green laser option for expanded utility and fluorochrome options? Simple push...
型號：
時間： 2019 - 04 - 17
描述The 2018 Attune NxT Acoustic Focusing Cytometer has expanded functionalities to drive your research forward. Designed and developed to remove common barriers associated with flow cytometry, the evolutionary capabilities of the Attune NxT flow cytometer offer adaptable optical configuration options so you can get the most out of your multicolor analysis.? Free Attune NxT software with researcher-inspired upgrades? New green laser option for expanded utility and fluorochrome options? Simple push...
型號：
時間： 2019 - 04 - 17
描述The 2018 Attune NxT Acoustic Focusing Cytometer has expanded functionalities to drive your research forward. Designed and developed to remove common barriers associated with flow cytometry, the evolutionary capabilities of the Attune NxT flow cytometer offer adaptable optical configuration options so you can get the most out of your multicolor analysis.? Free Attune NxT software with researcher-inspired upgrades? New green laser option for expanded utility and fluorochrome options? Simple push...
型號：
時間： 2019 - 04 - 17
描述The 2018 Attune NxT Acoustic Focusing Cytometer has expanded functionalities to drive your research forward. Designed and developed to remove common barriers associated with flow cytometry, the evolutionary capabilities of the Attune NxT flow cytometer offer adaptable optical configuration options so you can get the most out of your multicolor analysis.? Free Attune NxT software with researcher-inspired upgrades? New green laser option for expanded utility and fluorochrome options? Simple push...
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